Development of Dispersive Solid Phase Extraction Using Green Natural Sorbent for Enrichment of Antibiotics Prior to High Performance Liquid Chromatography Analysis

Abstract

This study used chia seed mucilage as a biosorbent with modified the surface area by iron(III)-natural reagent particles for dispersive solid phase extraction of tetracycline prior to HPLV-UV. Four tetracyclines (TCs), including oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC), and doxycycline (DC), were used with the optimal parameters for all experiments. The linear range was 2−500 µg L-1, with a coefficient larger than 0.9957. The limits of detection (LODs) and quantification (LOQs) were 0.7−2.0 µg L-1 and 2.0−7.0 µg L-1, respectively. Precisions were shown in the form of relative standard deviation (RSD%) values of less than 9.1% and 6.9% for intra-day and inter-day. For the first time, alternative biosorbents was successfully quantified TCs residues in milk samples, with recoveries ranging from 80.2 to 109.4%. The analytical performance of enrichment factors (EFs) was satisfactory in the 55-105 range. Furthermore, the selected biosorbent materials and the proposed method were praised for their natural mucilage and reagent, eco-friendly biopolymer, biodegradability, nontoxicity, cheap cost, lower solvent consumption, and reduced extraction procedure.

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