Chemical Constituents from the Natural Fruiting Bodies of Serpula dendrocalami C. L. Zhao

Abstract

The natural fruiting bodies of Serpula dendrocalami C. L. Zhao have been chemically investigated for its bioactive compounds. In this research, the natural fruiting bodies of Serpula dendrocalami were collected from Nakhon Phanom Province, Northeastern Thailand. The living culture was isolated, grown on PDA, and preserved at the Department of Biology, Faculty of Science, Mahasarakham University (MSUCC032). This fungus was identified as Serpula dendrocalami on the basis of the ITS rDNA gene sequencing data. Biological activity and chemical study of this species have never been reported. The extract from dichloromethane was subjected to purify by silica gel and Sephadex LH-20 column chromatography. Chemical investigation led to the isolation of three undescribed O-prenyl-tyrosine oxime derivatives serpulanines D−F (8−10) together with five known compounds, serpulanine C (3), valencic acid (11), 4-prenyloxyphenylacetic acid (12), ergosterol (13), and ergosterol endoperoxide (14). The structures were elucidated on the basis of NMR spectroscopic and mass spectrometry data. The E-configuration of oxime group was proposed based on their spectroscopic data compared with serpulanine C from previous report. The undescribed compounds 8−10 have been evaluated for their antibacterial activities against Gram-positive pathogenic bacteria, methicillin-resistant Staphylococcus aureus (MRSA), methicillin-sensitive Staphylococcus aureus (MSSA), and Bacillus cereus and cytotoxicity against A549 cell lines. These compounds were inactive in antibacterial and cytotoxic activities assays.

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