Please use this identifier to cite or link to this item: http://202.28.34.124/dspace/handle123456789/2895
Title: The use of MONTANIDE™ IMS 1312 VG PR Adjuvant on the Immune Response of Nile tilapia (Oreochromis niloticus) to an Inactivated Streptococcus agalactiae Vaccine by Immersion Vaccination
การใช้ MONTANIDE™ IMS 1312 VG PR เป็นสารเสริมฤทธิ์ในวัคซีนเชื้อตายของเชื้อ Streptococcus agalactiae ในการกระตุ้นการตอบสนองทางภูมิคุ้มกันในปลานิล (Oreochromis niloticus) โดยการให้วัคซีนแบบแช่
Authors: Piyachat Phudinsai
ปิยะฉัตร ภูดินทราย
Eakapol Wangkahart
เอกพล วังคะฮาต
Mahasarakham University
Eakapol Wangkahart
เอกพล วังคะฮาต
eakapol.w@msu.ac.th
eakapol.w@msu.ac.th
Keywords: MONTANIDE™ IMS 1312
โรคสเตรปโตคอคโคซีส
สารเสริมฤทธิ์
การพัฒนาวัคซีน
ระบบภูมิคุ้มกันแบบเยื่อเมือก
Group B streptococcus (GBS)
MONTANIDE™ IMS 1312
Streptococcal disease
Adjuvanted vaccine
Vaccine development
Mucosal immune response
Group B streptococcus (GBS)
Issue Date:  26
Publisher: Mahasarakham University
Abstract: Streptococcus agalactiae is a common pathogenic bacterium caused of streptococcosis, which has a negative impact on Nile tilapia aquaculture. Numerous vaccines have been recently developed to combat this disease, which are key components of global health efforts to prevent disease outbreaks. MONTANIDE™ IMS 1312 is a micro-emulsion recommended for immersion of fish. However, the data on the effectiveness of those immersion vaccines containing this aqueous adjuvant in fish are limited. The objective of this research was to explore the potential of MONTANIDE™ IMS 1312, an adjuvant for immersion vaccination, administered with an S. agalactiae inactivated whole-cell vaccine (SAIV) in Nile tilapia. Fishes were separated into three groups: 1) fish were vaccinated by immersion vaccination with PBS (CTRL), 2) fish were vaccinated by immersion vaccination with SAIV vaccine alone (SAIV), and 3) fish were vaccinated by immersion vaccination with SAIV containing MONTANIDE™ IMS 1312. We found that the activity of several innate immunity parameters was increased significantly (P < 0.05) following the immunization. As expected, the levels of specific IgM antibody were significantly increased post-vaccination, and the highest IgM antibody levels were found in the fish vaccinated with SAIV containing MONTANIDE™ IMS 1312. Analysis of the transcriptional expression of major pro-inflammatory cytokines, as well as the presence of IgM+ B cells, revealed significant increases, suggesting that Nile tilapia were able to initiate cellular immune responses following vaccination. Taken together, our results indicate that using MONTANIDETM IMS 1312 in combination with a SAIV can induce strong protection post S. agalactiae infection. Importantly, administration of an adjuvanted immersion vaccine is safe, no side effects were observed, and it does not negatively impact fish growth. In conclusion, MONTANIDE™ IMS 1312 has the potential to be used as adjuvants for immersion vaccines against streptococcosis in Nile tilapia.
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URI: http://202.28.34.124/dspace/handle123456789/2895
Appears in Collections:The Faculty of Technology

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